Bovine lactoferrin (LFLTF) instruction manual This kit uses double antigen sandwich enzyme-linked immunoassay (ELISA) to determine the human epidemic encephalitis antibody IgG (JE IgG) in the specimen. The microplate is coated with purified human JE antibody IgG (JE IgG) antigen to make a solid-phase antigen, which can be combined with JE IgG antibody in the sample and washed After removing unbound antibody and other components, it is combined with HRP-labeled Japanese encephalitis antibody IgG (JE IgG) antigen to form an antigen-antibody-enzyme-labeled antigen complex. After thorough washing, the substrate TMB is added for color development . TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm and compared with the CUTOFF value to determine the presence or absence of human epidemic encephalitis antibody IgG (JE IgG) in the specimen.
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