This kit is for research use only. Detection range: 96T2.5μmol / L -80μmol / L Purpose: This kit is used to determine the content of nitric oxide synthase (NOS) in rat serum, plasma and related liquid samples. Experimental principle This kit uses the double antibody sandwich method to determine the level of rat nitric oxide synthase (NOS) in the specimen. Microporous plates were coated with purified rat nitric oxide synthase (NOS) antibody to make solid-phase antibodies. Nitric oxide synthase (NOS) was added to the monoclonal antibody-coated microwells in turn, followed by HRP-labeled Nitric oxide synthase (NOS) antibody binds to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with nitric oxide synthase (NOS) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of rat nitric oxide synthase (NOS) in the sample was calculated by a standard curve. Kit composition 1 30-fold concentrated washing solution 20ml × 1 bottle 7 stop solution 6ml × 1 bottle 2 enzyme label reagent 6ml × 1 bottle 8 standard product (160μmol / L) 0.5ml × 1 bottle 3 enzyme label coated plate 12 well × 8 strips 9 standard diluent 1.5ml × 1 bottle 4 sample diluent 6ml × 1 bottle 10 instruction manual 1 copy 5 developer A solution 6ml × 1 bottle 11 sealing film 2 sheets 6 developer B solution 6ml × 1 / Bottle 12 sealed bag 1 specimen requirement 1. Specimens are extracted as soon as possible after collection, and extraction is performed according to relevant literature, and experiments should be conducted as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 ℃, but repeated freezing and thawing should be avoided. The samples containing NaN3 cannot be detected because NaN3 inhibits horseradish peroxidase (HRP) activity. Procedure 1. Dilution of standard product: This kit provides one original standard product. The user can perform dilution in a small test tube according to the following chart. 80μmol / L No. 5 standard 150μl original standard added 150μl standard dilution 40μmol / L No. 4 standard 150μl standard No. 5 added 150μl standard dilution 20μmol / L No. 3 standard 150μl No. 4 standard Add 150μl standard dilution 10μmol / L No. 2 standard 150μl No. 3 standard add 150μl standard dilution 5μmol / L No. 1 standard 150μl No. 2 standard add 150μl standard dilution 2. Add sample: Separately set blank wells (the blank control wells do not add samples and enzyme label reagents, the rest of the operation is the same), standard wells, sample wells to be tested. Accurately add 50μl of the standard on the enzyme-coated plate, add 40μl of sample diluent to the sample well, and then add 10μl of the sample to be tested (the final dilution of the sample is 5 times). Add the sample and add the sample to the bottom of the well of the microplate, try not to touch the wall of the well, shake gently to mix. 3. Incubation: Seal the plate with a sealing plate and incubate at 37 ° C for 30 minutes. 4. Mixing solution: Dilute the 30-fold concentrated washing solution with distilled water 30-fold and reserve for use. 5. Washing: Carefully remove the sealing film, discard the liquid, spin dry, fill each well with the washing solution, and let it stand for 30 seconds before discarding , Repeat this 5 times, pat dry. 6. Add enzyme: add 50μl of enzyme label reagent to each well, except blank well. 7. Incubation: The operation is the same as 3. 8. Washing: The operation is the same as 5. 9. Color development: add 50μl of developer A to each well, then add 50μl of developer B, mix gently, and develop color at 37 ° C in the dark 15 minutes. 10. Stop: Add 50μl of stop solution to each well to stop the reaction (the blue color turns to yellow at this time). 11. Determination: Measure the absorbance (OD value) of each well in sequence with the blank air conditioner at zero and 450 nm wavelength. The measurement should be carried out within 15 minutes after adding the stop solution.
PLLA Filler
Both hyaluronic acid dermal filler and Reborn PLLA filler are designed to address lost volume, fine lines, folds and wrinkles. Due to the aging process, environmental stressors, and our genetic predisposition, we suffer from loss of collagen at a rate of about 1% annually after the age of 20. Loss of collagen leads to thinning of skin which results in the formation of wrinkles and folds. Collagen is a fibrous protein in the deep dermal layer that gives our skin structural support and helps to maintain shape, moisture, and elasticity of the skin. A healthy supply of collagen is the source of smooth skin and a youthful appearance.
But ordinary Dermal Fillers are made of Hyaluronic acid designed to hold its shape in the form of a gel-like substance. hyaluronic acid dermal fillers do not stimulate collagen production, but rather are meant to temporarily replace missing volume. The effects of Dermal Filler are apparent immediately. Whereas the effects of Reborn PLLA Dermal Filler happens gradually since it takes time for the body to build collagen.
Reborn PLLA dermal Filler is an injectable biostimulator made of Poly-l-lactic Acid (PLLA). Reborn PLLA has a liquid consistency and it's injected underneath the skin. Once injected, Reborn incorporates into the tissues and stimulates your body's natural production of collagen.Reborn plla is a non-surgical treatment that requires minimal downtime, enabling you to return to your daily activities after the procedure. Reborn gives beautiful and natural-looking results that will appear gradually avoiding sudden dramatic changes in your appearance. Reborn's main ingredient Poly-l-lactic acid(PLLA), provides results that last more than two years.
Over the course of several months, the drug biodegrades and is eliminated from the body, but in its place what remains is not emptiness, but collagen and elastin. That is why the effect of Reborn injections lasts for up to two years or longer!
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