First make up 3 kinds of stock solution, then make up the dyeing solution. See the table below for details
Kinds of ingredients, configuration method Storage time A liquid takes 3g basic fuchsin dissolved in 100mL of 70% ethanol can be stored for a long time Liquid B takes 10mL of liquid A, add 90mL of 5% carbolic acid (also known as phenol) aqueous solution for use within 2 weeks Take 55mL of solution B in solution C, add 6mL of each of acetic acid and formalin (formaldehyde aqueous solution), take the original solution of C20-30mL, add 70% to 80mL of 45% acetic acid, and add 1.8g of sorbitolThis solution has the advantages of convenient staining with magenta acetate, and the advantage that Schiff reagent only stains nuclei and chromosomes, and the staining effect is stable and reliable. This solution is suitable for chromosomes of various sizes of animals and plants, somatic cell chromosomes and meiotic chromosomes, and has a fairly strong staining performance, good preservation, and does not deteriorate at room temperature for two years. Similar to Carnot's solution.
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