Cleaning ELISA kit materials:
1. Distilled water.
2. Sampler: 5ul, 10ul, 50ul, 100ul, 200ul, 500ul, 1000ul.
3. Oscillator and magnetic stirrer, etc.
Precautions for operation of ELISA kit:
1. Reagents should be stored according to the label instructions and returned to room temperature before use. The sparse standards should be discarded and cannot be stored.
2. The slats not used in the experiment should be immediately returned to the packaging bag, sealed and stored to avoid deterioration.
3. Other unused reagents should be packed or covered. Do not mix reagents of different batches. Use before warranty.
4. Use a disposable pipette tip to avoid cross-contamination, and avoid using a sampler with a metal part when drawing the stop solution and the substrate A and B liquids.
5. Use a clean plastic container to configure the washing solution. Mix all components and samples in the kit thoroughly before use.
6. When washing the enzyme plate, it should be fully patted dry. Do not put the absorbent paper directly into the reaction well of the enzyme plate to absorb water.
7. Substrate A should evaporate to avoid opening the lid for a long time. Substrate B is sensitive to light and avoid prolonged exposure to light. Avoid contact with hands, it is toxic. The OD value should be read immediately after the experiment is completed.
8. The order of adding reagents should be consistent to ensure that all reaction plates are incubated for the same time.
9. Carry out the incubation operation according to the time, the amount and sequence of the liquid added in the manual.
Safety: 1. Avoid direct contact with the stop solution and substrates A and B. Once exposed to these liquids, rinse with water as soon as possible.
2. Do not eat, drink, smoke or use cosmetics in the experiment.
3. Do not use your mouth to absorb any components in the kit.
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