Quantitative test kit (ELISA) for rat total antioxidant capacity (T-AOC)
user's Guide
[Rat Total Antioxidant Capacity (T-AOC) Elisa Kit Kit Name]
Quantitative test kit (ELISA) for rat total antioxidant capacity (T-AOC)
[T-AOC Elisa kit kit usage]
Quantitative detection of total antioxidant capacity (T-AOC) in rat serum, plasma and related liquid samples.
[Test Principle of Total Antioxidant Capacity of Rat (T-AOC) Elisa Kit]
This kit uses double-antibody two-step sandwich enzyme-linked immunosorbent assay (ELISA). Add the standard and the test sample to the pre-coated rat total anti-oxidation capacity (T-AOC) monoclonal antibody transparent enzyme label coated plate. After incubation for a sufficient time, wash to remove unbound components, and then add the enzyme Standard working solution, after incubation for a sufficient time, wash to remove unbound components. Substrates A and B were added in sequence, and the substrate (TMB) was converted into a blue product under the catalysis of horseradish peroxidase (HRP), which turned yellow under the action of acid. The concentration of oxidation capacity (T-AOC) is positively correlated. The OD value is measured at a wavelength of 450 nm. According to the OD value of the standard and the sample, the total antioxidant capacity (T-AOC) content of the sample is calculated.
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