Problem 1: The image quality of the microscope has deteriorated for a period of time, and the image quality will be significantly improved after turning it off for a period of time.
Diagnosis: The lens coating technology is not enough, and the coating will diverge after the lens is heated for a long time.
Problem 2: The image is clear when manually focusing, and the image is blurred after releasing the hand.
Diagnosis: The focusing mechanism is aging.
Trouble 3: The image is clear during eyepiece observation, but the captured image is not clear. When the collected image is adjusted clearly, the observation image in the eyepiece is not clear again.
Diagnosis: The system has insufficient parfocality, and observation and acquisition cannot be synchronized.
Trouble 4: The stage slides and shifts.
Diagnosis: The locking mechanism of the stage uses a star gear, which reduces the stability of long-term use.
Problem 5: The clear edges in the middle of the image are blurred.
Diagnosis: Incorrect spherical aberration correction.
Problem 6: The microscope field is clear in the middle, but why is the edge blurred?
Diagnosis: It is a problem of the objective lens. Generally, the achromatic objective lens has poor flat field performance. 60-80% of the center of the field of view is generally clear, but the surrounding will be blurred. The solution is to change to a plan achromatic objective.
Trouble 7: What should I do if the background of the fluorescence microscope eyepiece and the photo are not dark enough?
Diagnosis: You can shake out the condenser of the transmitted light when taking a fluorescent picture, otherwise there will be a reflection image when looking at the fluorescence. There is another method. If the fluorescence is strong enough, the aperture stop of the fluorescence can be adjusted appropriately to remove the background a little. In addition, there is a black balance option in the software, which can make the background darker.
Problem 8: The fluorescence range of the fluorescence microscope becomes smaller, and the 40x objective lens cannot see clearly?
Diagnosis: There are many possibilities for such symptoms, such as:
1. The ND filter is not fully in place. At this time, you will see that part of it is bright and part is not bright, and the bright part and the non-bright part are roughly a circular arc;
2. The diaphragm is closed. At this time, you should see that the middle bright edge is dark or there is no fluorescence; if the center of the diaphragm is not correct, it is not the middle bright, it may be a certain place bright; at this time, you see The bright part is probably a regular polygon;
3. The center of the mercury lamp is not correct: it may be that the center has not been adjusted after replacing the new bulb, or that the curiosity research has vividly adjusted the adjustment rod on the light box. At this time, the partially bright shape is not easy to describe, and various situations are possible. Yes, but it will not be basically the same as the shape of 1, 2. You can basically see the clear boundaries of the rules in the former two. In this case, the clear boundaries of the rules are basically not seen; 4. The excitation block turntable is not in place Or the splitting prism is not in place: this possibility is relatively small, and the resulting image deviation is similar to case 1;
Fault 9: The fluorescence is particularly weak during observation. What are the solutions?
diagnosis:
1 The fluorescent dye and the fluorescent block of the microscope may not match well
2 The center of the mercury lamp is not adjusted well, or the life of the mercury lamp is up
3 Whether the production of samples can be better,
4Whether a suitable objective lens is used
Fault 10: How can the dark field observation of the microscope be achieved?
Diagnosis: Microscope observation can be observed in bright field, dark field, polarized light, phase difference, etc., different microscopes use different observation methods
1. Remove the bright field condenser, and install the dark field condenser on the condenser bracket;
2. Make the objective lens enter the optical path;
3. Open the aperture diaphragm;
4. Put the sample on the stage to focus;
5. Remove the eyepiece from the eyepiece tube, observe the edge of the objective lens from the observation tube, and adjust the knobs on both sides of the condenser lens to center the dark field ring;
6. Insert the eyepiece into the eyepiece tube and observe the dark field image obtained;
7. Move the condenser up and down until uniform dark field illumination is achieved.
Problem 11: How to adjust the mercury lamp center of a fluorescent microscope?
Diagnosis: To adjust the center of the fluorescent mercury lamp, first of all, we first understand the basic structure of the fluorescent microscope light box.
1 Light box structure
Lamphouse (Lamphouse Frame): A mechanical support structure that fixes the internal structure of the fluorescent light box, which has a good heat dissipation effect.
Light bulb (Mercury Arc Lamp): a high-pressure short-arc mercury lamp, a tube that produces a continuous spectrum, and provides illumination for fluorescence experiments.
Mirror: It is similar to a mirror structure with a bowl opening towards the mercury lamp. Because the light emitted by the mercury lamp bulb is in a scattered direction, in order to ensure better lighting efficiency, this light collection device is added to collect the light emitted by the mercury lamp in the opposite direction of the illumination.
Collector lens: The purpose is to collect the light obtained by the mercury lamp and the reflector to form a high-efficiency parallel beam, which is provided to the fluorescent lighting system.
Understand the structure of the light source, let's explain how to adjust the mercury lamp
2 How to adjust the mercury lamp
1) If there is an adjustment accessory, use the accessory adjustment. If not, you can put a flat white paper on the sample holder to focus 3? C [Kq
2) Adjust the collector focus knob to adjust the main image and the mirror image clearly (below), you will find two images at this time, but their positions are one up or one down or one left and one right
3) Adjust the mercury lamp position adjustment knob to adjust the two images to overlap the two images
4) At this time, the center has been adjusted, you can take samples for observation. If the brightness is not uniform in the field of view, you can fine-adjust the focus knob.
Fault 12: Trouble shooting and solution of common faults of CCD camera.
diagnosis:
1. No video signal output after power on. First check whether the polarity of the external power supply is correct and the voltage meets the requirements.
2. The color distortion may be due to the improper setting of the white balance switch (AWB2), or it may be that the ambient lighting conditions have changed too much. At this time, you should check whether the switch setting is in the OFF position, and try to improve the ambient lighting conditions.
3. The phenomenon of distorted or geometric distortion of the image may be the problem of the geometric correction circuit of the camera and the monitor, or the problem of the optical lens, or the characteristic impedance of the connecting cable or device does not match the output impedance of the camera . When the above phenomenon occurs, please check the optical lens used first, and then check whether the characteristic impedance of the cable or other connected equipment is 75Ω.
4. Several black bars appear in the vertical direction of the picture. Generally, the ripple of the DC power supply voltage is too large. Filtering should be strengthened and a DC power supply with good performance should be used.
5. There are multiple vertical bars in the vertical direction of the screen. This may be caused by the reflection caused by the mismatch of the external video cable or the characteristic impedance of the device and the camera. Pay attention to whether the characteristic impedance of the selected video cable and other connected processing equipment is 75Ω.
6. The screen noise may be caused by the following reasons: · The internal circuit board of the camera is poorly grounded; · The video amplitude output is not enough; · The ambient light is not enough; Whether the connection of the optical cable is poor or there is a short circuit; · The monitor itself has a poor signal-to-noise ratio .
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